@article {4211e7e0aa03431395a02d7c7e6e3332,
	title = {Initiation of fibronectin fibrillogenesis is an enzyme-dependent process},
	journal = {Cell Reports},
	volume = {42},
	number = {5},
	year = {2023},
	note = {Publisher Copyright: {\textcopyright} 2023},
	publisher = {Cell Press},
	abstract = {Fibronectin fibrillogenesis and mechanosensing both depend on integrin-mediated force transmission to the extracellular matrix. However, force transmission is in itself dependent on fibrillogenesis, and fibronectin fibrils are found in soft embryos where high forces cannot be applied, suggesting that force cannot be the sole initiator of fibrillogenesis. Here, we identify a nucleation step prior to force transmission, driven by fibronectin oxidation mediated by lysyl oxidase enzyme family members. This oxidation induces fibronectin clustering, which promotes early adhesion, alters cellular response to soft matrices, and enhances force transmission to the matrix. In contrast, absence of fibronectin oxidation abrogates fibrillogenesis, perturbs cell-matrix adhesion, and compromises mechanosensation. Moreover, fibronectin oxidation promotes cancer cell colony formation in soft agar as well as collective and single-cell migration. These results reveal a force-independent enzyme-dependent mechanism that initiates fibronectin fibrillogenesis, establishing a critical step in cell adhesion and mechanosensing.},
	keywords = {Cell Adhesion, CP: Cell biology, fibronectin fibrillogenesis, lysyl oxidaze family, mechanosensing},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2023.112473},
	author = {Shay Melamed and Shelly Zaffryar-Eilot and Elisabeth Nadjar-Boger and Rohtem Aviram and Huaning Zhao and Wesal Yaseen-Badarne and Rotem Kalev-Altman and Dalit Sela-Donenfeld and Oded Lewinson and Sophie Astrof and Peleg Hasson and Haguy Wolfenson}
}
